identification of high molecular weight proteins in aegilops crassa from iran

نویسندگان

مهسا بام نشین

پردیس کشاورزی و منابع طبیعی دانشگاه تهران محمدرضا نقوی

پردیس کشاورزی و منابع طبیعی دانشگاه تهران محمد جعفر آقایی

مؤسسه تحقیقات نهال و بذر، بخش ژنتیک علیرضا طالعی

پردیس کشاورزی و منابع طبیعی دانشگاه تهران محمد اسماعیل حسنی

چکیده

in this study, 120 aegilops crassa (mmdd genome) accessions from iran were evaluated for high molecular weight proteins. based on sds-page analysis, 17 bands were selected and mass spectrometry was used for protein identification. among 17 bands selected for maldi-tof-tof-ms analyzing, only 4 bands were identified as high molecular weight glutenin subunit similar to aegilops ventricosa and triticum aestivum with high probability. moreove, 2 bands as beta-amylase similar to hordeum vulgare were detected and 11 of them had no ms/ms data. the results showed that the iranian accessions of aegilops crassa have favorable glutenin subunits that are useful in improving bread wheat quality.

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